Fig. 3: NPM1–TYK2 fusion kinase is an oncogenic driver in lymphoid cell transformation.
From: Functional characterization of NPM1–TYK2 fusion oncogene
A Fusion kinase-dead mutant blocks kinase activation and downstream signaling. Cell lysates were made from Ba/F3-vector, Ba/F3–NPM1–TYK2, and Ba/F3–NPM1–TYK2–K462R cell lines, and immunoblot analysis was performed for FLAG (NPM1–TYK2), phospho-NPM1–TYK2, phospho-STAT1/3/5, and respective total proteins. β-Actin served as an internal control. B Fusion kinase signaling drives cell transformation. Ba/F3-Vector, Ba/F3–NPM1–TYK2, and Ba/F3–NPM1–TYK2–K462R cells were grown in RPMI medium in the absence of IL-3. Cell viability from each condition was determined daily by trypan blue exclusion assay. Points represent the mean of three independent experiments; bars represent SEM. ∗∗∗P < 0.0005 was considered as statistically extremely significant.
