Fig. 3: S-Adenosylmethioninamine and acetylated polyamine flux contribute to diacetylspermine production in ex vivo TNBC-PDX tissue slices. | npj Precision Oncology

Fig. 3: S-Adenosylmethioninamine and acetylated polyamine flux contribute to diacetylspermine production in ex vivo TNBC-PDX tissue slices.

From: Pharmacometabolomics reveals urinary diacetylspermine as a biomarker of doxorubicin effectiveness in triple negative breast cancer

Fig. 3

Quantified urea cycle (a), polyamine (b) and acetylated polyamine (c) metabolites. d Atom transition map of 13C-glucose (e), 15N-methionine (f), and 15N-arginine (g), stable isotope metabolic flux in TM97 and TM98 ex vivo tissue slices treated with vehicle (DMSO) or 1 µM doxorubicin for 24 h. Experiments were performed in triplicate. Data are presented as mean ± s.e.m., n = 6. Significance was determined by two-sided one-way ANOVA with Tukey’s test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs TM97; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs TM98 control.

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