Fig. 2: Analysis of a DNA-Sequencing library identified two FGFR2 implicated translocations.

A Circos plot illustrating complex fusion between chromosomes 1 (SAMD13), 4 (G3BP2), and 10 (FGFR2). Additional fusions are also indicated, and a complete annotation of fusions within this model can be found in the supplementary file. Each arc represents a predicted gene fusion event, where the width of the arc is proportional to the strength of the evidence supporting the prediction. Blue arcs represent fusions between chromosomes and red arcs represent fusions within chromosomes. B DNA-Seq identified a complex translocation where chr10:121,482,464 is joined to chr1:84,303,484, placing FGFR2-exon 17 (including the 3’UTR, indicated by a thinner rectangle) upstream of SAMD13-exon 2 and chr4:75,709,417 is joined to chr10:121,482,522, placing FGFR2-exon 16 upstream of G3BP2-exon 1. C Schematic of full length FGFR2 (red), full length G3BP2 (blue), and FGFR2-G3BP2 fusion. The 8-amino acids translated from the G3BP2 5’UTR are represented by the gray wavy line. Forward and reverse PCR primers used for RT-PCR are shown in green arrows. Ig I/II/III Immunoglobulin-like domain I/II/III, TM transmembrane domain, NTF2-L nuclear transport factor 2-like domain, IDR 1/2/3 Intrinsically Disordered Domain 1/2/3, RRM, RNA-Recognition Motif. D Western blot showing that FGFR2 in the PDC and PDO models has a higher molecular weight compared to wildtype FGFR2 control, supporting the existence of the FGFR2-G3BP2 fusion.