Fig. 7: Effects of CXCR4 on proliferation, migration, and invasion of CSCs. | npj Precision Oncology

Fig. 7: Effects of CXCR4 on proliferation, migration, and invasion of CSCs.

From: The regulatory role of cancer stem cell marker gene CXCR4 in the growth and metastasis of gastric cancer

Fig. 7

A Flow cytometry sorted CD44+ and CD44- MKN45 cells; B RT-qPCR detected gene expression of CXCR4 in CD44+ and CD44- MKN45 cells; C RT-qPCR detected the knockdown efficiency of CXCR4 in CD44 + MKN45 cells; D Western blot detected the knockdown efficiency of CXCR4 in CD44 + MKN45 cells; E Colony formation assay detected the proliferation ability of each group of CD44 + MKN45 cells; F Transwell assay detected the migration and invasion ability of each group of CD44 + MKN45 cells, scale bar = 50 μm. * indicates a significant difference compared to the sh-NC or CD44- group, *p < 0.05, **p < 0.01, ***p < 0.001. Each cell experiment was repeated three times.

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