Table 1 Characteristics of patients enrolled in the study

Patient ID	Sex	Age	Mutation^a	Disease sites	Stage IV subcategory	Best response (RECIST)	EP	Progression sites	Survival status	Number of samples
1	F	75	p.V600E	Brain, lymph nodes, lung	M1d	PR	N	Brain	Dead	3
3	F	81	p.V600K	Liver	M1c	SD	N	Liver	Dead	3
6	F	42	p.V600E	Peritoneum	M1c	PR	N	Peritoneum	Dead	4
8	M	85	p.V600E	Brain, liver, lymph nodes, lung	M1d	PR	N	Brain, lung, soft tissues, lymph nodes	Dead	3
9	F	37	p.V600E	Soft tissue, lymph nodes, liver, adrenal gland, bones, peritoneum	M1c	PR	N	Soft tissues, liver, heart	Dead	4
10	F	65	p.V600K^b	Brain, lymph nodes, lung, liver	M1d	PR	N	Brain	Dead	4
12	M	59	p.V600E	Lymph nodes	M1a	CR (SR)	N	NA	Alive	3
13	F	42	p.V600E	Brain, peritoneum, lymph nodes, liver, soft tissues, lung, adrenal gland	M1d	PR	Y	Brain	Dead	4^c
15	F	46	p.V600E^b	Lung, bones	M1c	PR (SR)	N	NA	Alive	3
17	M	48	p.V600E	Soft tissues, thoracic wall, bones, subcutaneous, abdominal wall	M1c	CR	Y	Bones, soft tissues, peritoneum, lung	Dead	2
19	M	64	p.V600E	Lung, lymph nodes	M1b	PD	Y	Brain, lung, spleen, lymph nodes	Dead	2
24	M	51	p.V600E^b	Tonsils, lymph nodes	M1c	CR (SR)	N	NA	Alive	3
27	M	85	p.V600K	Lymph nodes, liver	M1c	PR	N	Liver, lung, adrenal gland, spleen	Dead	4
28	M	84	p.V600E	Subcutaneous, lymph nodes, liver, bones	M1c	PR	N	Adrenal gland, brain	Dead	3
34	F	75	p.V600K^b	Lung	M1b	PD	Y	Peritoneum, lung	Dead	1
48	M	59	p.T599dup	Pleura, lung, lymph nodes	M1b	PR	N	Pleura, liver, lymph nodes, brain	Alive	3
49	M	64	p.V600E	Brain, liver, lung, lymph nodes, bones	M1d	PD	Y	Brain	Dead	1

SD stable disease, PR partial response, CR complete response, SR still responding, PD progressive disease, EP early progression, i.e. progression free survival ≤6 months, Y yes, N no, NA not appropriate, Number of samples total number of plasma samples collected for every patient.
^aBRAF mutation identified in the liquid biopsy if not otherwise indicated.
^bBRAF mutation identified in the FFPE tissue sample.
^cAdditional samples corresponding to therapy change after progression and one month later were analyzed⁴⁶.

Search