Fig. 3: Integrative quantification of regulated cell death levels in cancers and normal tissues. | npj Precision Oncology

Fig. 3: Integrative quantification of regulated cell death levels in cancers and normal tissues.

From: Pan-cancer evaluation of regulated cell death to predict overall survival and immune checkpoint inhibitor response

Fig. 3

a The 18 regulated cell death signatures in this study. Three genes, including TP53, BCL2, and BAX were screened out in 9 RCD types. b The circle heat map shows the average of the RCD score in individual cancer types. The average RCD score, cancer types, and tissue types were shown from the outer to the inner side. The RCD score was scaled by zero-mean normalization (Z score). c The heat map shows the RCD score and the ssGSEA scores of individual samples in TCGA. The RCD score was defined as the sum of the ssGSEA score of the 18 RCD. The right panel was the bar plot showing the sum of the ssGSEA score of all samples in TCGA. d The right panel shows the proportion of ssGSEA score of each RCD across the cancers in TCGA. The middle panel shows the RCD type, which had the highest proportion across the cancers. The left panel shows the RCD types with the highest proportion of RCD types among all cancers. For example, of a total 30 cancers, disulfidptosis had the highest proportion of 18 RCD types in 18 cancer types. e The heat map shows the Spearman correlation between any two of the 18 RCD types for the 30 cancers. CD 1 contained 18 types of RCD with the exception of pyroptosis, and CD 2 contained 18 types of RCD with the exception of alkaliptosis. f The bubble plot shows the Spearman correlation of the RCD score and the ssGSEA score of the single RCD type. The p values < 0.05 were considered significant, while the opposite was considered non-significant. g Average RCD score across the normal tissues in the GTEx databank. The darker the blue, the larger the sample size. h In most cancers, compared with adjacent normal solid tissue (blue), the RCD score in primary tumors (red) was higher, with utilization of the Wilcoxon rank sum test. **** represented p < 0.0001. *** represented p < 0.001, ** represented p < 0.01. * represented p < 0.05. i Compared with the normal brain cortex, the RCD score of the LGG, GBM, and glioma (LGG-GBM) was significantly higher in the GTEx and TCGA databases. And the RCD score increased with grade. For Wilcoxon rank sum test, “****”, “***”, “**”, “*” represented p < 0.0001, p < 0.001, p < 0.01, <0.05, respectively.

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