Fig. 4: Construction of CRISPR/Cas12a-based AIGS.
From: Combination of MRI-based prediction and CRISPR/Cas12a-based detection for IDH genotyping in glioma
a Simplified schematic diagram of the optical system, heating system, and fluorescence detection subsystems of the AIGS system. b Schematic diagram of the optical path in the optical system. c Internal structure of the instrument. d Images of the AIGS system. e A typical detection result from AIGS. The results can be observed by monitoring fluorescence curves. f Test results using negative samples under the same conditions. g A cohort of frozen tumor samples was assayed for IDH mutation by AIGS (n = 20).
