Fig. 2: Spatial patterns of immune infiltration.

a Six representative ROIs are shown for each of the spatial phenotypes; diffuse, focal and insignificant immune infiltration, three from each of the two TMA slides. Boundary of circular 300 µm diameter ROIs are shown on top, with segmented tumor/immune or tumor only AOIs below (red=PanCK, green = CD45, blue = Syto13). The segmentation masks are colored in teal (tumor) and lime (immune) for TMA1, and in pink (tumor) and lime (immune) for TMA2. b Tumor and immune protein signatures in spatial phenotypes of immune-insignificant and infiltrated tumors. Tumor segments in regions of insignificant immune infiltration had higher levels of CD25 and Fibronectin, while infiltrated tumor regions had higher CD45, PD-L1, HLA-DR, CD44, IDO1 and CD11c. c Immune segments from regions with low immune cell ratio were higher in CTLA-4, FOXP3 and PD-L2, while regions with high immune cell ratio were higher in CD3, beta-2-microglobulin (B2M), CD4, CD45, CD45RO, CD8, CD44, and STING. d Regions with diffuse immune infiltration had tumor segments that were higher in IDO1, PD-L1, B2M, CD45, and Tim-3, compared to regions with focal immune infiltration. e Immune segments of diffuse infiltration patterns were higher in IDO1, granzyme B (GZMB) and CD3, while focal immune segments were higher in CD163. Differential expression was assessed through linear mixed models (LMM) with Patient ID as random effect, including only malignant tumors. Significance (-log10 p-value) was plotted against LMM regression coefficient. Red dotted line marks p = 0.05.