Fig. 4: Tumor immune phenotypes in OC subtypes.

a Diffuse, focal and insignificant immune infiltration were present in Type 1 and Type 2 malignant OC, as well as in benign and borderline samples. b Distribution of diffuse, focal and insignificant immune infiltration ROIs across histology subtypes of OC (malignant samples only). c gdc and (d) ccr across histotypes. ROIs are colored by patient IDs. Boxplots display median value (center line), first (lower hinge) and third (upper hinge) quartiles. Whiskers extend to the largest and smallest values, respectively. e, f Linear Mixed Models (LMM) with Patient ID as random effect and Type 1/2 as fixed effect to identify differences in immune infiltration between Type 1 and Type 2 OC in (e) tumor AOIs, and (f) immune AOIs. Only malignant tumors were included. Significance (-log10 p-value) were plotted against LMM regression coefficients. Red dotted line marks p = 0.05. g Biomarkers upregulated in low grade histotypes of OC as compared to all other samples and to other Type 1 histologies, respectively. Comparisons were made using LMM with Patient ID as random effect and histology as fixed effect. Biomarker significantly higher in each histotype are listed. Clear cell carcinoma is not included as no biomarkers were identified for that histotype.