Fig. 1: Discovery of the fusion gene - PUM1-TRAF3. | npj Precision Oncology

Fig. 1: Discovery of the fusion gene - PUM1-TRAF3.

From: PUM1-TRAF3 fusion protein activates non-canonical NF-κB signaling via rescued NIK in biliary tract cancer

Fig. 1

a Workflow for fusion analysis in biliary tract cancer(BTC). b Schematic representation of PUM1-TRAF3 fusion, involving PUM1 (PUF domain, chromosome 1) and TRAF3 (MATH domain, not the RING and Zinc finger domains; chromosome 14). Schematic diagrams and circos plots were visualized using Arriba. In the index patient (patient 1 (B01 or P1)), the chimeric junction between PUM1 and TRAF3 for PUM1-TRAF3 fusion was confirmed via Sanger sequencing. c Fluorescent in situ hybridization (FISH) with two probes for PUM1 (red) and TRAF3 (green) in the P1 tissue slide. Arrow, fused signal. d In situ proximal ligation assay (PLA) was performed to visualize PUM1-TRAF3 protein expression in the P1 tissue slides using PUM1 rabbit polyclonal antibody and TRAF3 mouse monoclonal antibody; spot-like PLA signals were observed in the sample from the patient (B01 or P1) expressing the fusion protein (PUM1-TRAF3, red; DAPI, blue). e ddPCR product (P1, P5, P11, P12, and P36) was confirmed via Sanger sequencing. f FISH was performed with PUM1 (red) and TRAF3 (green) probes in the P5, P11, P12, and P36 tissue slides. Arrow, fused signal.

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