Fig. 3: LINE-1 ORF1p is expressed and released by CCOC cell lines. | npj Precision Oncology

Fig. 3: LINE-1 ORF1p is expressed and released by CCOC cell lines.

From: LINE-1 ORF1p expression occurs in clear cell ovarian carcinoma precursors and is a candidate blood biomarker

Fig. 3

A ORF1p expression (WB) in hEM3 (endometrial cell line), 12Z (endometriotic cell line), and a panel of clear cell ovarian carcinoma (CCOC) cell lines. B ORF1p expression (green) by immunofluorescence in CCOC. Scale bars: 10 µm. C ORF1p detection (WB) in conditioned media from benign and CCOC cell lines. Coomassie blue was used as loading control. D Size distribution profile of extracellular vesicles (EVs) characterized via nanoparticle tracking analysis. Bar plot indicates the average size (nm) of EVs in each cell line. Data are shown as mean ± SD. E ORF1p detection by Simoa assay in conditioned media and F sEVs. Bar plots indicate the average level of ORF1p (pg/mL) in each sample. Data are shown as mean ± SD.

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