Fig. 6: Validation of the effect of MCAM on osteosarcoma progression. | npj Precision Oncology

Fig. 6: Validation of the effect of MCAM on osteosarcoma progression.

From: Single-cell and spatial transcriptomics reveals the key role of MCAM+ tip-like endothelial cells in osteosarcoma metastasis

Fig. 6

A Violin plot of MCAM in EC subtypes. B Violin plot of the expression of MCAM in different sample groups. C Prognostic analysis of patients with high and low MCAM expression. D Representative immunofluorescence images of MCAM in osteosarcoma tissue and peritumoral tissue (n = 2). E PCR detection of siRNA-MCAM knockdown efficiency. F Representative images of tube formation assay. G Number of connections of cell-forming tubes. H Total length of cell-forming tubes. (NC negative control, PC paracancerous, PT primary tumor, Scale bar = 200 μm,***p < 0.001, ****p < 0.0001).

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