Fig. 2: CEACAM6 influence on proliferation in lung cancer cells. | npj Precision Oncology

Fig. 2: CEACAM6 influence on proliferation in lung cancer cells.

From: Cytokine and epigenetic regulation of CEACAM6 mediates EGFR-driven signaling and drug response in lung adenocarcinoma

Fig. 2

A qRT-PCR (upper) and immunoblotting (lower) analysis of CEACAM6 expression in HCC827 cells transduced with the lentiviral vector encoding shCEACAM6 or scramble control. ***P < 0.001. B Annexin V assay measuring apoptotic cells in HCC827 cells transduced with shCEACAM6 or scramble control. **P < 0.01. C GSEA from RNA-seq data identifying differential gene expression involved in the regulation of epithelial-cell-proliferation pathway (left) and epithelial-cell-proliferation pathway (right) in CEACAM6-high (top 100 cases) versus CEACAM6-low (lowest 100 cases) expression in adenocarcinomas from TCGA-LUAD. D Immunoblotting analysis assessing p-ERK1/2 and p-AKT expression in HCC827 cells transduced with shCEACAM6 or scramble control. Quantification was performed using ImageJ software. E Dot-plot analysis to assess the correlation between p-ERK1/2 (pT202Y204) and CEACAM6 expression by RPPA profiling and RNA-seq from the TCGA-LUAD database (n = 357). F Correlation analysis evaluating the epithelial cell proliferation signature levels in CEACAM6-high (top 50 cases) versus CEACAM6-low (lowest 50 cases) expression in adenocarcinomas from TCGA-LUAD. The epithelial cell proliferation signature was derived from Gene Ontology63.

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