Fig. 3: Disease progression within RET cysteine pathogenic variants is driven in part by NF-κB. | npj Precision Oncology

Fig. 3: Disease progression within RET cysteine pathogenic variants is driven in part by NF-κB.

From: Kinome profiling reveals pathogenic variant specific protein signalling networks in MEN2 children with Medullary Thyroid Cancer

Fig. 3

a Network map of kinases within the NF-κB pathway (CHUK = IKKalpha. IKBKE = IKKepsilon). b Differential kinase activity of kinases shown in a Data shown is Z-scored. c p65 H-DAB immunohistochemistry of patient thyroid tissue. Labels show pathogenic variant, patient number and total number of samples for the pathogenic variant group. For images on the left, the scale bar shows 500 µm and the hashed line boxes show the location of images on the right. The scale bar for images on the right represents 100 µm. d Quantification of p65 cellular levels within thyroid tissue across all pathogenic variant groups. Colours show patient histology groups (group 1 (Histologically normal) orange, group 2 (C-cell hyperplasia) pink, group 3 (Medullary thyroid cancer) blue, group 4 (metastatic Medullary thyroid cancer) green). Analysed by One-way ANOVA followed by Brown-Forsythe test, * p < 0.05, ** p < 0.01, *** p < 0.001. e Data shown in d with Cysteine variants split by their metastatic potential C609-620X moderate, C634X high. Analysed by Mann-Whitney test.

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