Fig. 1: Knockdown of PGAM2 promotes proliferation and tumor growth of HCC cells both in vitro and in vivo.

A PGAM2 levels in HCC cell lines were detected by western blot. B Detection of PGAM2 protein and mRNA levels by western blot and RT-qPCR in PGAM2 overexpression cells and controls. C–E Cell growth rates and colony formation abilities detection of PGAM2 overexpressing cells and controls. F–H Detection of PGAM2 protein and mRNA levels by western blot and RT-qPCR in PGAM2 knockdown cells and controls. I–K Cell growth rates and colony formation abilities detection of PGAM2 knockdown cells and controls. L–N Representative image, volume and weight of xenografts with PGAM2 knockdown cells or controls (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001.