Fig. 4: PGAM2 suppresses aerobic glycolysis and key enzymes expression in HCC cells. | npj Precision Oncology

Fig. 4: PGAM2 suppresses aerobic glycolysis and key enzymes expression in HCC cells.

From: Hepatocellular carcinoma cells downregulate PGAM2 via SIRT2-mediated deacetylation modification to enhance aerobic glycolysis

Fig. 4

A Schematic representation of aerobic glycolysis and key enzymes involved. B–G Detection of glucose consumption and lactate production in PGAM2 overexpressing cell lines and controls; PGAM2 knockdown cell lines and controls; PGAM2-WT and PGAM2-K100R expressing cell lines; and cell lines overexpressing Vector, PGAM2, SIRT2 alone or in combination. H mRNA levels of key glycolytic enzymes were detected by RT-qPCR in HCCLM3 cell lines expressing PGAM2-WT and PGAM2-K100R. I Protein levels of key enzymes were detected by western blot in PGAM2-WT and PGAM2-K100R expressing cell lines; PGAM2 overexpressing cell lines and controls; PGAM2 knockdown cell lines and controls. *p < 0.05, **p < 0.01, ***p < 0.001, ns no significance.

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