Fig. 5: Knockdown of PGAM2 enhances glycolysis by promoting STAT3-mediated LDHA expression in HCC cells. | npj Precision Oncology

Fig. 5: Knockdown of PGAM2 enhances glycolysis by promoting STAT3-mediated LDHA expression in HCC cells.

From: Hepatocellular carcinoma cells downregulate PGAM2 via SIRT2-mediated deacetylation modification to enhance aerobic glycolysis

Fig. 5

A–C Detection of lactate production, growth rate, and colony formation ability of HCC cell lines with PGAM2, LDHA overexpression or knockdown. D Schematic model of STAT3 binding sites to LDHA promoter. E ChIP-qPCR analysis of LDHA promoter region binding to STAT3 in SNU449 cell line. IgG is included as the control for IP. F Dual-luciferase reporter assay to identify the binding of STAT3 to LDHA promoter. G, H Detection of p-STAT3 in PGAM2 overexpressing and knockdown HCC cell lines by western blot. I, J Protein and mRNA levels of LDHA were detected by western blot and RT-qPCR in controls and PGAM2 knockdown cells treated with or without STAT3 inhibitor stattic. **p < 0.01, ***p < 0.001.

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