Fig. 3: Detection of ofCS + CTCs in patients with advanced cancer.
From: Multi-cancer detection of circulating tumor cells by targeting oncofetal chondroitin sulfate
a The number of ofCS+ CTCs detected with rVAR2:dextran in 4 mL of blood in patients with epithelial cancer (n = 25) or mesenchymal cancer (n = 3) and in healthy controls (n = 13). The samples were stained with PE-labelled rVAR2:dextran and APC-conjugated antibodies targeting CD45, CD66b and CD16. Cell nuclei were stained with DAPI. b Representative immunofluorescence images of ofCS+ CTCs from six different cancer patients. The CTCs were stained as described in (a). A CTC was defined as ofCS(+), CD45(−), CD66b (−), CD16(−) and DAPI(+).
