Fig. 2: Bulk SA analysis of secreted proteins in HNSCC tissues using thermodynamic methodology revealed unbalanced processes in HNSCC that are closely linked to T-cell infiltration and improved immunotherapy response.
From: The responses of HNSCC patients to immunotherapy are shown by two novel co-expression patterns
A, C Amplitudes \(({\lambda }_{\alpha }\left(k\right))\) of process 1 (Act+) and 2 (Inf+) were computed (Methods) for each patient (n = 3 samples per patient) and presented for each tissue. Patients 4698 and 4950 harbor both processes, while 4905 only Act+. B, E Cytokines with significant weights (Gi, “Methods”) were co-expressed and grouped into unbalanced processes using Gi values and STRING protein–protein connections. The circle radius corresponds to the Gi value of each protein i. The sign of \({\lambda }_{\alpha }\left(k\right)\) (A, C) determines correlation/anti-correlation, or separation, between the samples (Methods): Blue-labeled proteins are upregulated in patients with blue-labeled amplitudes \(\left({\lambda }_{\alpha }\left(k\right)\right)\), and red-labeled proteins are upregulated in patients with red-labeled amplitudes. D Inf phenotype differentiates between HPV+ and HPV− samples (T-test **p value < 0.01).
