Fig. 5: Reprogramming of neutrophils in PDAC. | npj Precision Oncology

Fig. 5: Reprogramming of neutrophils in PDAC.

From: Single-cell transcriptome analysis identifies MIF as a novel tumor-associated neutrophil marker for pancreatic ductal adenocarcinoma

Fig. 5

A, B Images of IF staining for OLR1+ and MIF+ TANs in human PDAC tissues are presented. Nuclei were stained with DAPI. The scale bar is 20 μm. C Schematic workflow of the orthotopic pancreatic tumor model and PDX model. Neutrophils from bone marrow, spleen, and pancreatic tumors of tumor-bearing mice were used for flow cytometry. D, E Representative flow cytometry plots illustrating MIF expression in Ly6G+ neutrophils within bone marrow, spleen, and tumor. MIF expression specifically identified a distinct subset of tumor-infiltrating neutrophils. F Experimental setup involving the in vitro culture of sorted neutrophils from wild-type mouse bone marrow in cDMEM compared with TCM. G Representative flow cytometry plots showing that the expression of MIF increased in sorted neutrophils after 2 days of culture in TCM but not in control medium. H Neutrophils cultured in TCM upregulated MIF over time. Line plots show the percentage of MIF+ cells in the neutrophil population. Schematic diagrams were created with BioRender.com. *p < 0.05; **p < 0.01; ***p < 0.001. PDX patient-derived xenograft, TCM tumor-conditioned medium.

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