Fig. 6: MIF+ TANs promoted PDAC progression through the activation of the ERK and AKT pathways and EMT. | npj Precision Oncology

Fig. 6: MIF+ TANs promoted PDAC progression through the activation of the ERK and AKT pathways and EMT.

From: Single-cell transcriptome analysis identifies MIF as a novel tumor-associated neutrophil marker for pancreatic ductal adenocarcinoma

Fig. 6

PDAC cells (AsPC-1, PANC-1, and CFPAC-1 cells) were cocultured with MIF-overexpressing dHL-60 cells or control cells. A To evaluate the proliferative ability of PDAC cells, CCK-8 assays were performed. B, C EdU assays were performed to evaluate the proliferative ability of PDAC cells. D, E Colony formation assays were performed to evaluate the proliferative ability of PDAC cells. F, G Wound healing assays were conducted to detect the migratory ability of PDAC cells. The scale bar is 100 μm. H, I Transwell assays were conducted to detect the migratory ability of PDAC cells. The scale bar is 100 μm. J–L The expression of ERK, p-ERK, AKT and p-AKT in PDAC cells was detected by western blotting. M The expression of EMT markers (E-cadherin, vimentin, and N-cadherin) in PDAC cells was detected by western blotting. The data are representative of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.

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