Fig. 2: Differential cell abundance and differential gene expression between BCG naïve and BCG recurrent NMIBC.

A Differential abundance analysis was performed using the proportion of cells per sample comparing naïve and recurrent samples (Wilcoxon test, no p-values met significance threshold of p < 0.05). B Heatmap of enriched hallmark pathways from GSEA analysis of differentially expressed genes per cell type between naïve and recurrent samples. The color scale represents the normalized enrichment score (NES) which met p-value threshold of p < 0.05. Non-significant NES scores were not plotted (white). Positive NES values (red) indicate pathway enrichment in recurrent samples. C Log2 fold change of leading-edge genes for the interferon gamma (IFNG) pathway (red = enriched in recurrent, blue = enriched in naïve).