Fig. 2: Interaction dynamics of nick translocation.

Plot of velocity (left axis, red curve), center of mass (CoM) of the nicked-DNA (right axis, blue curve), and the number of translocated nucleotides (far right axis, black curve) during the entire translocation time of the nicked-DNA. The purple line in the center of the plot indicates the location of the graphene nanopore membrane. One clearly observes the CoM of nicked-DNA starts above and ends below the line. The area marked in green shows the interaction dynamics between nick and graphene: reduction in velocity, stalled CoM of nicked-DNA, and standstill count of the number of translocated nucleotides between 10 and 11 bases of nicked-DNA (location of the nick).