Extended Data Fig. 5: Catalytic pockets of the HNH domains.
From: Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
a–c, Close-up view of Mg2+-binding catalytic pockets of the HNH domains of St1Cas9 (a), Nm1Cas9 (b) and AnCas9 (c). Mg2+ and water molecules were coloured green and red, respectively. d, In vitro DNA cleavage assay of the wild type and various mutants of St1Cas9. The linearized plasmid containing a target sequence was used as the substrate and the full-length sgRNA was used as the guide RNA in the assay.
