Extended Data Fig. 7: The different substitutions in the dienophiles have an influence on both overall activities and stereoselectivities.
From: Enzymatic control of endo- and exo-stereoselective Diels–Alder reactions with broad substrate scope
The enzymatic reactions were performed using different dienophiles (200 μM) and diene 8 (200 μM) as substrates in the presence of 3 μg MaDA-3 or its variants at the optical conditions for 10 min. The red and blue columns indicate the exo/endo selectivities and dienophile conversions respectively. Enzyme activity values represent mean ± standard deviation (s.d.) of three independent replicates.
