Extended Data Fig. 2: Chemo-enzymatic syntheses of Diels-Alder type natural products using MaDA-1 and MaDA-2/3.
From: Enzymatic control of endo- and exo-stereoselective Diels–Alder reactions with broad substrate scope
All enzymatic products of MaDA-1 were determined as endo products since their NMR data and optical rotations are consistent with those of the Diels-Alder type natural products with endo configuration. Similarly, all enzymatic products of MaDA-2 and MaDA-3 were determined as exo products. The ee values for all the enzymatic products were determined as >99%. The detailed protocols and chiral HPLC analysis were described in the chemical synthesis part below. Note: chemo-enzymatic syntheses of all the D-A products with exo configuration were achieved using MaDA-3 except mulberrofuran J and albafuran C.
