Extended Data Fig. 1: Overview of the IsPETase directed evolution progression.
From: Directed evolution of an efficient and thermostable PET depolymerase
The crystal structures represent an overview of the evolution, with the IsPETaseTS protein represented as a turquoise ribbon. The catalytic triad and W185 are shown in ball and stick representation coloured by all atoms, with blue and grey carbon atoms, respectively. The three mutations in IsPETaseTS compared to IsPETaseWT are highlighted as yellow spheres, mutations installed in directed evolution rounds 1-2 as light blue spheres, the rationally inserted additional disulphide bridge (DSB) as black spheres and mutations installed in rounds 3–6 as pink spheres. More details on the evolution outcomes can be found in Supplementary Table 1.
