Extended Data Fig. 2: Comparison of reactions with IsPETaseTS and HotPETase over a range of temperatures.
From: Directed evolution of an efficient and thermostable PET depolymerase
48 h time-courses of cryPET reactions, showing the mean total concentration of released MHET and TPA, with HotPETase (yellow-pink) and IsPETaseTS (blues) over time, using 0.4% cryPET substrate loading (4 g L−1) and 0.29 mg g−1 enzyme loading (0.04 μM). Reactions were performed in pH 9.2, 50 mM Gly-OH buffer, 4% BugBuster, in triplicate, with error bars representing the s.d. of the replicate measurements.
