Extended Data Fig. 6: Interactions between QDs and microbes studied by flow cytometry.
From: Maximizing light-driven CO2 and N2 fixation efficiency in quantum dot–bacteria hybrids
a and b, Dispersion of pure CdTe QDs. c and d, Microbial culture of X. autotrophicus. The microbe–semiconductor hybrids at (e and f) t = 0 hr and (i and j) t = 24 hrs after assembly. The mixture of QDs and microbes at (g and h) t = 0 hr and (k and l) t = 24 hrs after assembly. a, c, e, g, i, and k are the plots of SSC-A versus FSC-A that illustrate the identity of the microbial population. b, d, f, h, j, and l are the plots of emission at 525 ± 50 nm versus FSC-A that illustrate the distribution of microbial population gated in the SSC-A/FSC-A plot attached with emissive QDs. The circles in the plots illustrate the gated areas corresponding to the microbial populations attached with emissive QDs whose temporal trends were shown in (m). Representative results presented above (n = 3 biological replicates with 10,000 particles recorded before gating, error bars present the standard deviation). Additional quantification of the percentage of QDs closely interacting with microbes are available in Supplementary Fig. 4.
