Extended Data Fig. 5: Photocrosslinking of diazirine-labelled albicidin (photo-Albi) to the DNA. | Nature Catalysis

Extended Data Fig. 5: Photocrosslinking of diazirine-labelled albicidin (photo-Albi) to the DNA.

From: Molecular mechanism of topoisomerase poisoning by the peptide antibiotic albicidin

Extended Data Fig. 5

a. Structures of N-terminal photo-Albi and control compound. b. DNA cleavage assay with Mu217 fragment and indicated concentrations of compounds. The labelled DNA band appearing upon irradiation of a photo-compound when bound to the complex is marked by a yellow star. c. Ciprofloxacin competition experiment. Pre-incubation with excess CFX prevents albicidin binding and crosslinking. Details of the experiment are available in Methods section.

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