Extended Data Fig. 8: Inhibition of supercoiling by Albi-1/2/3.
From: Molecular mechanism of topoisomerase poisoning by the peptide antibiotic albicidin
a. Chemical structures of Albi-1, Albi-2 and Albi-3. b. Example supercoiling inhibition assay gels: Albi-1, decreasing concentrations (320, 160, 80, 40, 20, 10, 5, 2.5 nM); Albi-2 (320, 160, 80, 40, 20 nM); Albi-3 (960, 800, 640, 480, 320, 160, 80, 40 nM). First lane - relaxed pBR322; second lane - DNA gyrase. 40 nM albicidin was used as positive control (third lane). Different concentrations were tested and representative gels are shown.
