Table 2 Data collection statistics
From: Molecular mechanism of topoisomerase poisoning by the peptide antibiotic albicidin
Dataset | EcGyr–Mu217–albicidin | EcGyr–Mu217-Albi-1 | EcGyr–Mu217–Albi-2 |
|---|---|---|---|
Microscope | Titan Krios G3i | Titan Krios G3i | Titan Krios G3i |
Magnification | 105,000× | 105,000× | 105,000× |
Voltage (kV) | 300 | 300 | 300 |
Electron dose (e− Å−2) | 39.08 | 41.57 | 39.59 |
Detector | K3 | K3 | K3 |
Defocus range (−µm) | 2.1–0.9 | 3–0.9 | 2.4–0.9 |
Pixel size (Å) | 0.86 | 0.86 | 0.86 |
Symmetry imposed | C1 | C1 | C1 |
Micrographs (no.) | 14,469 | 8,136 | 11,579 |
Initial particle images (no.) | 2,040,353 | 1,366,620 | 1,222,762 |
Final particle images (no.) | 147,019 (core) | 37,902 (TG) | 49,921 |
23,435 (insert) | 25 663 (AA) | ||
22 162 (XT) | |||
Global map resolution (Å) | 2.6 (core) | 3.25 (TG) | 3.25 |
3.30 (AA) | |||
3.06 (insert) | 3.41 (XT) | ||
Fourier shell correlation (FSC) threshold | 0.143 | 0.143 | 0.143 |
