Extended Data Fig. 6: 34S labelling experiments. | Nature Catalysis

Extended Data Fig. 6: 34S labelling experiments.

From: Radical S-adenosyl-l-methionine FeS cluster implicated as the sulfur donor during albomycin biosynthesis

Extended Data Fig. 6

(a) ESI-MS spectrum of S-(bimane)2 obtained by reacting mBBr with the denatured 34S-reconstituted ΔRS-cluster AbmM variant after another round of PT treatment. (b) ESI-MS spectrum of S-(bimane)2 obtained by reacting mBBr with the denatured 34S-reconstituted wildtype AbmM (32S-PT-34S-AbmM, RS-labelled). (c) ESI-MS spectrum of 14 generated from the single-turnover reaction of 10 with 34S-reconstituted wildtype AbmM (32S-PT-34S-AbmM, RS-labelled). The 34S enrichment was determined after correcting for natural abundance of 34S.

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