Fig. 3
From: Triplicate parallel life cycle divergence despite gene flow in periodical cicadas

Maximum-likelihood (ML) tree resulting from the concatenated sequences for individual cicadas. The tree was made ultrametric with the time of most recent common ancestor (tMRCA) of all Magicicada set to 3.89 million years ago6 (the 95% confidence interval of this node is shown by the broken line). Numerals above the branches are the bootstrap percentages resulting from the ML analysis. Estimated ages and 95% confidence intervals based on two calibrations using the upper and lower confidence limits of Magicicada tMRCA are shown for five major nodes (grey bars)