Fig. 2
From: mTORC1 impedes osteoclast differentiation via calcineurin and NFATc1
Early mTORC1 deletion in HSCs impairs osteoclast precursor proliferation and survival. Analyses of Raptorfl/fl-Vav1-iCre cKO mice and littermate controls. a Images of the pups at 6 weeks of age (n = 2). b Pup body weight at 6 weeks of age (n = 2). c Representative image of spleen at 6 weeks of age. d Quantification of spleen/body weight ratio at 6 weeks of age (n = 2). e Representative image of bone length at 6 weeks of age. f Total bone marrow cells in 6 weeks old mice (n = 2). g, h Expression of osteoclast markers (g) and osteoclastogenic transcription factors (h) in bone marrow osteoclast differentiation cultures (n = 6). Rosi rosiglitazone. i Representative images of TRAP-stained bone marrow osteoclast differentiation cultures. j Osteoclast resorptive activity measured by calcium release from bone plates (n = 8). k Raptor expression in bone marrow osteoclast differentiation cultures (n = 6). l Osteoclast precursor proliferation by BrdU incorporation (n = 10). m Caspase 9 expression in bone marrow osteoclast differentiation cultures (n = 6). Error bars, SEM; *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001; n.s. non-significant. R raptor
