Fig. 1
From: Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin
Physicochemical properties of OMD. a SDS-PAGE of OMD and DCN. b SEC-MALS of OMD and DCN. Solid lines represent absorbance at 280 nm (blue: OMD, orange: DCN). Dotted lines represent molecular weight (blue: OMD, orange: DCN). c X-ray crystal structure of OMD. The N-terminal and C-terminal regions of OMD are depicted on the left and right side of the panel, respectively. d Superimposition of OMD (cyan) on DCN dimer (gray and orange). The structure of DCN was obtained from the PDB (entry code 1XEC). e Interface residues at the DCN dimer (gray and orange). The structure of OMD (cyan) has been superimposed on one of the chains of the DCN dimer. The figure evidenced the different character of several residues of OMD at the position where residues of DCN make important contributions to the dimer interface. Yellow dotted lines represent H-bonds between molecules of DCN. f Sequence alignment between OMD and dimeric SLRPs. Blue background represents identical residues among SLRPs. Red squares indicate residues involved in the dimerization of DCN and the SLRP protein biglycan (BGN)
