Fig. 4
From: Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin
Loss of the regulatory effect of OMD for collagen fibrils by mutations of E284 and E303 of OMD. a Direct binding assay between E284R/E303R mutant of OMD and type-I collagen using SPR. b Kinetic analysis of fibril formation with E284R/E303R in a dose-dependent manner (0–10 µM). c Distribution of fibril diameter in the absence and presence of OMD or E284R/E303R. The data in the absence and presence of OMD were obtained from our previous study11. The values of diameter were obtained from TEM images. ****P-value < 0.001, ns not significant (P-value > 0.05). d Fibril structure in the absence and presence of OMD or E284R/E303R using SEM. Bar, 500 nm
