Fig. 4
From: The paraspecific neutralisation of snake venom induced coagulopathy by antivenoms
The immunological cross-reactivity and neutralisation of Echis ocellatus and Dispholidus typus venom by antivenom is mediated by interactions with snake venom metalloproteinases. a Two-dimensional SDS-PAGE gel electrophoresis profiles of venom and their cross-reactivity with species-specific and paraspecific antibodies detected by western blotting. b Inhibition of venom and toxin-induced degradation of prothrombin by the metalloproteinase inhibitor EDTA. For both venoms and ecarin 1 mM of EDTA inhibits the complete degradation of prothrombin. Key: PM protein marker, FII prothrombin, V venom (ecarin for the ecarin experiment); numbers indicate the molarity of EDTA used in increasing concentrations from left to right. c The percentage immunodepletion of venom toxins bound by the antivenoms used in the antivenomic experiments. Comparisons of the immunodepletion of SVMPs with non-SVMP toxins demonstrate that the heterologous antibody preparations (e.g. SAIMR boomslang vs. Echis ocellatus; EchiTAbG and anti-ecarin vs. Dispholidus typus) predominately immunodeplete (bind to) SVMPs. Circles represent data points of the immunodepletion percentage of the individual toxins within each toxin group analysed (SVMPs or other toxins), and bars are additive for those data points found within each group. See also Supplementary Figs. 4 and 5
