Fig. 2

DARPP-32 regulates cell survival through Akt and Erk1/2. a A549, H1650 and H226 cells were transduced with control or DARPP-32 shRNAs. Cell lysates were collected and immunoblotted with antibodies against phosphorylated Akt (p-Akt; S473), total Akt (T-Akt), b phosphorylated Erk1/2 (p-Erk1/2), total Erk1/2 (T-Erk1/2), DARPP-32 and α-tubulin (loading control). c A549 and H226 cells were transduced with control, DARPP-32-, t-DARPP- or T34A DARPP-32-overexpressing clones. Phosphorylated Akt (p-Akt; S473), total Akt (T-Akt), d phosphorylated Erk1/2 (p-Erk1/2), total Erk1/2 (T-Erk1/2), DARPP-32 and α-tubulin (loading control) proteins were detected by immunoblotting of cell lysates. Densitometry of the indicated immunoblots was performed using ImageJ software. Each open circle on a graph represents an independent experiment. All immunoblots are representative of three independent experiments. Uncropped images of depicted immunoblots are shown in Supplementary Figs. 16, 17. All bar graphs represent mean ± SEM (n = 3). *P < 0.05, one-way ANOVA followed by Dunnett’s test for multiple comparison