Fig. 3
DARPP-32 positively regulates cell migration. a A549 and b H1650 cells transduced with lentivirus encoding control or DARPP-32 shRNAs were plated into 60 mm cell culture dishes, scratched and imaged at 0 and 14 h. c A549 and d H1650 cells infected with retrovirus encoding control, DARPP-32, t-DARPP or T34A DARPP-32 clones were scratched and imaged at 0 and 14 h. Dashed lines indicate the boundary of the edges of the wound at 0 h. Experiments were repeated at least three times in triplicate. Scale bar, 200 µm. Distance traveled by the migratory cells were calculated using ImageJ software. Each open circle on a graph represents an independent experiment. Results represent mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 and ****P < 0.0001, one-way ANOVA followed by Dunnett’s test for multiple comparison
