Fig. 5

Knockdown of IKKα and NF-ĸB2 reduces lung cancer cell migration. a A549 and H1650 cells transduced with lentivirus encoding control or IKKα shRNAs (clone numbers 04 and 05) were plated into 60 mm cell culture dishes, scratched and imaged at 0 and 14 h. b A549 and H1650 cells transduced with lentivirus encoding control or NF-ĸB2 shRNAs (clone numbers 04 and 05) were scratched and imaged at 0 and 14 h. c A549 and d H1650 cells expressing control, IKKα or NF-κB2 shRNAs were transfected with control (pMMP-LacZ) or DARPP-32 (pMMP-DARPP-32) overexpressing plasmids using Polyfect reagent. The cells were then scratched and imaged at 0 and 14 h. Dashed lines indicate the boundary of the edges of the wound at 0 h. Experiments were repeated at least three times in triplicate. Each open circle on a graph represents an independent experiment. Scale bar, 200 µm. Distance traveled by the migratory cells were calculated using ImageJ software. Results represent mean ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001, one-way ANOVA followed by Dunnett’s test for multiple comparison