Fig. 5 | Communications Biology

Fig. 5

From: Multiple claudin–claudin cis interfaces are required for tight junction strand formation and inherent flexibility

Fig. 5

Cis interface screening by evolutionary coupling and conservation analysis. a Intramolecular contact map obtained from the crystal structure of mCldn15 (PDB ID: 4P79) (gray) and the overlaid intermolecular contact map of mCldn15 identified by coevolutionary coupling analysis (red). The approximate locations of ECL1 and ECL2 and secondary structural elements are indicated along the axis, with TM domains in magenta, β-sheets in blue, and the ECH in orange. Predicted intermolecular residue-residue contacts were mapped onto the X-1 b and the three candidate interfaces Cis-1, Cis-2, and Cis-3 c obtained from HADDOCK. Intermolecular distance between the EC pairs are shown in green while intra-molecular distance between the same pair are shown in red. d Residue conservation of the X-1 and Cis-1 interface, in the region between ECH/ECL1 and ECL2. Side chains of residues within 3 Å of the two Cldn protomers are shown as sticks and color coded by their conservation ratio

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