Fig. 8 | Communications Biology

Fig. 8

From: Multiple claudin–claudin cis interfaces are required for tight junction strand formation and inherent flexibility

Fig. 8

The Cis-1-specific residue R79 in mCldn15 is involved in strand formation. a Cartoon representations of the mCldn15 Cis-1 interface highlighting residues S67, E157, and R79, and showing putative hydrogen bond interactions (dashed lines). b Confocal imaging of COS7 cells expressing mCldn15-GFP WT or the R79A mutant (green). Cellular actin was counterstained with Alexa 568-phalloidin (shown in magenta). c Freeze-fracture morphology of tight junction strands in HEK293T cells expressing mCldn15 R79A. Scale bar = 200 nm. d Confocal imaging of COS7 cells expressing mCldn15 mutants R79W, R79E, R79H, S67A, and S67A/R79A. e Percentage of transfected cell pairs that form tight junction at cell contacts for the mCldn15 WT and mutants. Scale bars = 5 µm for all confocal images, WT n = 104, R79A n = 106, R79W n = 202, R79E n = 143, R79H n = 402, S67A n = 150, S67A/R79A n = 150

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