Fig. 2
HSP70 and Plin2 mRNA expression in PBMCs are reduced after metabolic surgery. *** P<0.0001, **P<0.007. Data are mean ± s.e.m. or are expressed as median plus minimum and maximum values for whisker plots. a HSP70 mRNA expression in PBMCs from obese IR, NAFLD subjects (n = 14) before and after metabolic surgery, and in PBMCs from healthy controls (n = 12). b Plin2 mRNA expression in PBMCs from obese IR, NAFLD subjects (n = 14) before and after surgery, and in PBMCs from healthy controls (n = 12). c Correlation between HSP70 and Plin2 mRNA expressions in PBMCs from obese IR, NAFLD subjects before (R = 0.30, P = 0.044) and after surgery (R = 0.41, P = 0.014), and from healthy controls (R = 0.56, P = 0.0052). d Plin2 protein expression (median fluorescence intensity (MFI)) in monocytes and PBMCs from obese, IR subjects (n = 14) compared to healthy controls (n = 12) (CD14+CD16−: 86.77 ± 7.05 vs. 35.68 ± 4.57, P = 0.0001; CD14++CD16+: 98.72 ± 9.15 vs. 36.65 ± 4.59, P = 0.0001; CD14+CD16+: 86.52 ± 7.36 vs. 38.58 ± 4.79, P = 0.0006; total PBMCs: 86.60 ± 6.97 vs. 35.65 ± 4.41, P = 0.0001)
