Fig. 1

Zeb2 is expressed in developing midbrain. a In situ hybridization of Zeb2 combined with immunofluorescence staining of CXCR4 (green) and tyrosine hydroxylase (TH; blue) reveal the expression of Zeb2 in ventricular zone (VZ) and intermediate zone (IZ) in midbrain at E12.5. b, c, d Higher magnification images of boxed area in (a). Zeb2 mRNA is localized in VZ and IZ. c CXCR4 is localized in IZ and marginal zone (MZ). d Section was counterstained with DAPI. e Immunofluorescence shows that ZEB2 (magenta) protein is localized in VZ. f, g Higher magnification images of boxed area in (e) show ZEB2 (f; magenta) and NR4A2 (g; green). White arrowheads in f and g show ZEB2⁺/NR4A2⁺ cells, whereas open arrowheads show ZEB2⁺/NR4A2⁻ cells in IZ. h Immunofluorescence shows that ZEB2 (magenta) is not colocalized with CXCR4 (green). i, j Higher magnification images of boxed area in (h) show ZEB2 (i; magenta) and CXCR4 (j; green). k Immunofluorescence shows that ZEB2 (magenta) is not colocalized with PITX3 (green). Open arrowheads in (i, j) show ZEB2⁺/CXCR4⁻ cells in IZ. Scale bars in all panel: 50 μm. IZ, intermediate zone; MZ, marginal zone; VZ, ventricular zone.