Fig. 4

Overexpression of Zeb2 alters radial distribution and cell morphology in embryonic midbrain. a–c Immunofluorescence images show the distribution of GFP⁺ cells in midbrain floor plate at E13.5. Embryos were electroporated at E11.5, with pCAG-Egfp a, pCAG-Zeb2-Egfp b, or pCAG-Zeb2-Egfp + pCAG-Cxcr4-Egfp (c). d–f GFP⁺ cells overexpressing Zeb2 (e) show more multipolar morphology compared with monopolar/bipolar morphology in control GFP⁺ cells (d), whereas GFP⁺ cells co-electroporated with pCAG-Zeb2-Egfp + pCAG-Cxcr4-Egfp plasmids exhibit monopolar/bipolar morphology (f). g Percentage of GFP⁺ cells localized in MZ out of total GFP⁺ cells in midbrain floor plate of pCAG-Egfp (n = 4; median 68.9%, range 65.4–72.2%), pCAG-Zeb2-Egfp (n = 4; median 54.4%, range 52.1–55.8%) or pCAG-Zeb2-Egfp + pCAG-Cxcr4-Egfp (n = 4; median 69.5%, range 65.4–73.2%) electroporated midbrain. h Percentage of GFP⁺ cells displaying multipolar morphology in total GFP⁺ cells in VZ/IZ of pCAG-Egfp (n = 4; median 9.76%, range 8.55–10.4%), pCAG-Zeb2-Egfp (n = 4; median 20.9%, range 19.6–21.5%), or pCAG-Zeb2-Egfp + pCAG-Cxcr4-Egfp (n = 4; median 11.8%, range 10.9–12.6%) electroporated midbrain. Scale bars: 50 μm. Mann–Whitney U-test was used. IZ, intermediate zone; MZ, marginal zone; VZ, ventricular zone.