Table 1 Primers used for cloning of templates for riboprobes for in situ hybridization
From: A Zeb2-miR-200c loop controls midbrain dopaminergic neuron neurogenesis and migration
Gene | Sense primer (T7 underlined) | Antisense primer (SP6 underlined) | Reference Sequence |
|---|---|---|---|
Primary miR-200c | 5′-gagTAATACGACTCACTATAGGAAGGAGGAAGAGCGAGAGTG-3′ (124718826–124718807) | 5′-gagATTTAGGTGACACTATAGACATCATTACCCGGCAGTATTAGAG-3′ (124718326–124718349) | NC_000072.6 |
mZeb1 | 5′-gagTAATACGACTCACTATAGGCGTTGGCTCATTCTTAAGCTCT-3′ (3724–3745) | 5′-gagATTTAGGTGACACTATAGATGCTTCAGCTTCTCTCACAGTC-3′ (4342–4321) | NM_011546.3 |
mZeb2 | 5′-gagTAATACGACTCACTATAGGTAAGGGAGAGTGTTGTGGAG-3′ (620–639) | 5′-gagATTTAGGTGACACTATAGAGGGCTTCTGGGTAAATAATGG-3′ (895–875) | NM_001355289.1 |
