Fig. 3 | Communications Biology

Fig. 3

From: Facile carrier-assisted targeted mass spectrometric approach for proteomic analysis of low numbers of mammalian cells

Fig. 3

Comparison of SRM signal between 10 and 100 intact HMEC cells isolated from serial dilution measured by cPRISM-SRM: a XICs of transitions monitored for LVVVGAGGVGK derived from H/K/NRAS. 478.3/743.4 (blue), 478.3/644.4 (purple), 478.3/545.3 (chestnut). b XICs of transitions monitored for SFADINLYR derived from NRAS. 549.8/864.5 (blue), 549.8/793.4 (purple). c Quantification of EGFR pathway proteins in 100 intact HMEC cells by cPRISM-SRM. Detected proteins are in green, undetected proteins are in blue, unmeasured proteins are in orange. Activating interaction are shown as arrows, inhibiting interactions are shown as ‘T’ lines, and protein–protein interactions are shown as diamonds. Size of each node is directly proportional to protein abundance from our previous bulk HMEC measurement

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