Fig. 2

Characterization of 1-cell arrest phenotype caused by severe DD concentrations (2.5 µg mL⁻¹). a Percentage of fertilized embryos assessed by the formation of polar bodies do not show significant differences (P-value = 0.432) between DMSO-control (blue circles in the plot) (b) and DD-treated embryos (orange circles in the plot) (c), in which moreover no morphological differences in the presence of internal granules nor surface rugosity are observed. Maternal Wnt11 signal appeared asymmetrically distributed, mostly accumulated near the cell membrane (yellow arrowheads) that will form the prospective posterior vegetal pole both in DMSO-control (d) and DD-treated embryos despite the absence of the first cleavage (e), suggesting that DD does not alter the cellular architecture of the oocyte that drives primary axial polarity. Number of analyzed embryos (n) and number replicates (r) are indicated on top of each treatment. Reported means and standard deviations are represented in the plot with horizontal line and error bars, respectively. Scale bar 50 µm