Fig. 7
Detection of residual integration in NHP 4459 at 1 month post IDLV-1176 immunization. a Alu-PCR for vector integration and total-IDLV PCR were performed on serial dilutions of total DNA extracted from 1-month muscle biopsy of NHP 4459 (left) and genomic DNA extracted from CMMT-LV-Neo positive control (right). b Positive bands in Alu-PCR were extracted and cloned into pCR2.1 TOPO vector and subsequently sequenced using the M13F and M13R primers. The sequence alignment shows that the integrated sequence corresponds to IDLV-1176
